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HPLC and Ion Chromatography Co-
Looks can be Deceiving
What is it?
This is often visualised as peak tailing, apparent peak splitting, a seemingly single peak which is actually an extraneous peak/s from a previous analytical run or a seemingly single peak which is actually the elution of more than one analyte.
Why does it occur?
How can we check for Co-
A scientist must be certain that each chromatographic peak seen, represents a
single analyte from the current chromatographic run.
In order to clarify which peaks are the products of co-
For the purpose of co-
themselves very well to scanning chromatographic peaks as they elute,
providing that each analyte has its own discernible profile/spectrum.
These detectors include mass spectrometers, ultra-
scanning UV/Visible HPLC detectors, diode array, some
scanning fluorescence, and scanning voltammetry detectors.
These detectors may help to elucidate the Peak Purity of a
There are limitations, to the above detection methods,
particularly if one or more analytes are very closely related, if the spectra are similar, or if the analytes do not have
During method development another analytical confirmatory
technique, such as a mass spectroscopy, X-
diffraction and nuclear magnetic resonance etc., may be used to check the identity of each eluted peak.
Tips for better separation of Analytes within the Co-
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